Year 12 stress Essay Example | Topics and Well Written Essays - 2000 words

Year 12 stress - Essay Example In causing one’s death, the methods being used today are different but the intent and motivation are more or less the same. One of the frequently used methods involves trains and railroad tracks. Most of the suicide incidents reported in Japan these days involve students who flunked, or knew they would flunk, a high-stakes college admission test administered uniformly to graduating high school students nationwide. Japanese students have been raised to believe that their very lives and future ride on this battery of tests such that failure to pass it could mean the end of the world for them. That means abasement and dishonor to the proud Japanese. In the psychology of suicides, however, it is said that it is not enough that one’s sense of pride and honor is wounded to want to end it all. A strong instigating factor is stress which, psychologists say, comes from feeling out of control. So if an individual is in control of his senses, he might still seek ways to redeem his fallen honor and thus vindicate himself. Suicide thus becomes an attractive path only for people stressed out by the prospects of failure, which could be the reason for the alarming incidence of such cases among Japanese students. The same thing could be happening to Australian students in the past few years. A pressure-packed series of tests for pre-college students similar to Japan’s college admission tests has since the 1960s created the same sort of problem for public health and safety in Australia. Like the Japanese exams, a great deal of importance had been attached to the tests for Australia’s Higher School Certificate (HSC) that passing it has become a do-or-die proposition for the students involved. Too much is expected from students going through this examination that flunking it is considered out of the question. The HSC is

Restriction Fragment Pleomorphism Coursework Example | Topics and Well Written Essays - 1000 words

Restriction Fragment Pleomorphism - Coursework Example Restriction enzymes are highly specific in nature and single alteration in its recognize site (Restriction site) leads to complete inhibition of its activity. In RFLP technique particular gene having restriction site for an enzyme is used to generated DNA fragment(. If there was a mutation in this gene leads to altered restriction site which subsequently resultant in to DNA fragment having altered length. Based on size of a fragment and comparing with standard one can conclude mutation in given gene. Here in case of sample A three bands were obtained when digested with DdeI indicating heterozygous for Gal6V while XhoI digest indicates homozygous for Gal6V clearly indicating anomaly in the results as digestion with two different enzymes give exactly opposite results. Comparing the banding pattern of Gel1A with theoretical banding of standard, there should be 3bands on XhoI digest compared to single band on DdI in case of Gal6V homozygous. But the banding pattern obtained on image 1A was not fitting in to the criteria indicating abnormal preparation. thus all the interpretation were drawn from gel 1B. in case of sample B there was three bands found corresponding to homozygous for CD5(-CT) standard sample while it was found to be heterozygous for GAL6V. Similarly sample C was found to be heterozygous for CD5(-CT) and heterozygous for Gal6V. Three control samples were given as standered namely C1 homozygus for Gal6V, C2- normal individual and C3 homozygus for CD5(-CT). Banding patte rn obtained after digestion with DdeI and XhoI was as shown in fig 1A and 1B. Tabel 1 indicates phenotype and genotype of given sample. Table 1: Summary of Genotype and Phenotype of given sample Sample DdeI enzyme XhoI enzyme Genotype Phenotype A Glu6Val / + CD5(-CT)/ CD5(-CT) Heterozygous Glu6Val and Homozygus CD5(-CT) Thalassemia disease carrier of sickle cell anemia fetus B Glu6V/+ CD5(-CT)/ CD5(-CT) Heterozygous Glu6Val and Homozygus CD5(-CT) Thalassemia disease carrier of sickle cell anemia fetus C Glu6Val / + CD5(-CT)/ + Heterozygous Glu6Val Heterozygus CD5(-CT) Carrier sickle cell Crrier CD5(-CT) Conclusion: Genotyping of given sample were carried out using RFLP technique. The main objective of this study was to identify and differentiate closely related genetic disorder namely sickle cell anemia and thalassemia. Experimental results have indicated that there was some anomaly in electrophoretic patterns and thus experiments has to be performed again be for came in to any conclusion. Similarly experiments have demonstrated both aspect of RFLP at on side the technique was found to be easy, rapid and sensitive while other side it was found that it has some limitations like repeatability, requirement of large